Nikolić, Biljana M. Ivanović, Stefan Simić, Katarina Mitić, Zorica S. Todosijević, Marina M. Mladenović, Katarina Tešević, Vele V. info:eu-repo/semantics/article ABSTRACT Spring needles of Abies pinsapo Boiss. contained 84 identifiable volatile compounds. Monoterpene hydrocarbons dominated the essential oil (86.2%), with β-pinene (34.8%), α-pinene (24.6%), and limonene + β-phellandrene (11.9%) as the major constituents. Stepwise discriminant analysis distinguished young needles from 1- and 2-year-old needles. The average nonacosan-10-ol content was 36.9%, with minimal differences among age classes. Needle n -alkanes ranged from C16 to C33 , dominated by C29 (19.02%), C23 (18.89%), C27 (17.62%), and C25 (13.01%). The most abundant n -alkanes were C23 in young, C27 in 1-year-old, and C29 in 2-year-old needles. The total Carbon Preference Index of n -alkanes ranged from 3.2 to 7.3 (mean 4.6), peaking in young needles (6.0). The total average chain length ranged from 24.7 to 27.2 (mean 26.2). Principal component analysis also separated young needles from older ones. eng Variability of Essential Oil and Wax in Needles of Abies pinsapo Boiss. (Pinaceae) All rights reserved application/pdf 123477 bytes Wiley Chemistry and Biodiversity volume: 22 https://unilib.phaidrabg.rs/o:8800 doi:10.1002/cbdv.202503117 ISSN: 1612-1872 2025