o:8664 Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs en Chlorate (ClO3-) is a monovalent inorganic anion obtained by deprotonation of chloric acid. Its widespread historic use (as an herbicide) and numerous present applications result in notable findings of chlorate residues in food commodities. The aim of this paper is to present validation data confirming the fitness for purpose of the modified “QuPPe” method for LC- MS/MS determination of chlorate in plant-origin foodstuffs with high water content. The validation study was conducted according to SANTE 11312/2021 in the form of a full initial validation on three matrices representative of the leafy vegetable group. The results of the key validation parameters demonstrate the fitness for purpose of the pro- posed method. Recovery values (96.5-105.3%), repeatability (4.1-16.2%), intermediate pre- cision (3.9-16.4%) and expanded measurement uncertainty (12.6-32.1%) are in compliance with the criteria laid down in the SANTE document. Use of a graphitized carbon column for the separation of chlorate introduces the necessary step of frequent preconditioning with pla- nar compounds e.g., chlorophyll. However, this process does not result in significant delays or extended run times if carried out regularly. Chlorate, LC-MS/MS, Validation, SANTE, Hypercarb™ 1552099 10.18485/meattech.2025.66.3.96 2025-12-09T11:48:56.451Z 44 yes 46 Srđan Stefanović Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs https://orcid.org/0000-0002-8011-5654 Čaba Silađi Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs person 10.18485/meattech.2025.66.3.96 Danka Spirić Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs person https://orcid.org/0000-0002-6008-7625 Radivoj Petronijević Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs person https://orcid.org/0000-0002-3901-3824 Aleksandar Bajčić Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs person https://orcid.org/0000-0003-2923-4137 Nikola Betić Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Performance evaluation of the LC-MS/MS method for determination of chlorates in plant-origin foodstuffs person https://orcid.org/0000-0002-7375-6752 Vedrana Jelušić Veterinary Office of Bosnia and Herzegovina, Maršača Tita 9A/II, Sarajevo, Bosnia and Herzegovina person application/pdf 450676 https://unilib.phaidrabg.rs/o:8664 no yes 16 70 92000001 71A01 Meat technology - Special Issue 66/3 2025 1-576 66 3 552 555 Kaćanskog 13, 11 000 Belgrade Institute of Meat Hygiene and Technology 2025-12-01 www.journalmeattechnology.com