o:8657 Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines en This study investigated the presence of residues of eleven coccidiostats in unmedicated feed intended for broilers in the withdrawal phase, with the aim of assessing potential cross- contamination during feed production. Between 2023 and 2024, a total of 341 feed sam- ples were analysed, using validated LC-MS/MS methodology, for amprolium, diclazuril, halofuginone, lasalocid, salinomycin, maduramycin, monensin, narasin, nicarbazin, robe- nidine, semduramycin. Detected concentrations ranged from below the limit of quantifica- tion to 85.0 mg/kg. Non-compliant samples were identified for diclazuril (2/243), lasalocid (1/328), salinomycin (12/329), maduramycin (11/324), monensin (6/324), narasin (6/315), nicarbazin (2/327), and robenidine (7/333). The highest detected concentrations per analyte were: diclazuril 0.133 mg/kg, lasalocid 1.33 mg/kg, salinomycin 85.0 mg/kg, maduramycin 0.834 mg/kg, monensin 25.30 mg/kg, narasin 58.15 mg/kg, nicarbazin 33.70 mg/kg, and robenidine 35.2 mg/kg. Amprolium, halofuginone, semduramycin were not detected in any feed sample. Overall, 42 out of 341 feed samples (12.3%) exceeded the maximum permitted levels, indicating insufficient cleaning of production lines and inadequate separation be- tween medicated and non-medicated feed. The occurrence of such residues in feed intended for broilers in the withdrawal phase poses a risk of non-compliant residues in edible tissues, highlighting the need for stricter hygiene protocols, improved quality control in feed mills, and continuous monitoring to safeguard food safety and public health. Coccidiostat, Residue, Feed, Carry-over, Broiler, Cross-contamination 1552099 10.18485/meattech.2025.66.3.91 2025-12-09T10:17:52.533Z 44 yes 46 Milenko Babić Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines https://orcid.org/0009-0006-6467-6757 Zoran Petrović Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person https://orcid.org/0000-0003-2016-5681 Nikola Borjan Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person https://orcid.org/0000-0003-4067-3755 Ognjen Krnjaja Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person https://orcid.org/0009-0005-2242-0457 Damjan Gavrilović Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person https://orcid.org/0009-0000-3786-496X Čaba Silađi Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person https://orcid.org/0009-0004-1933-3849 Sanin Tanković Food Safety Agency of Bosnia and Herzegovina, Kneza Višeslava bb, 88000 Mostar, Bosnia and Herzegovina Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines person application/pdf 393882 https://unilib.phaidrabg.rs/o:8657 no yes 16 70 92000001 71A01 Meat technology - Special Issue 66/3 2025 1-576 66 3 529 533 Kaćanskog 13, 11 000 Belgrade Institute of Meat Hygiene and Technology 2025-12-01 www.journalmeattechnology.com