Babić, Milenko Petrović, Zoran Borjan, Nikola Krnjaja, Ognjen Gavrilović, Damjan Silađi, Čaba Tanković, Sanin eng Institute of Meat Hygiene and Technology https://unilib.phaidrabg.rs/o:8657 doi:10.18485/meattech.2025.66.3.91 Meat technology - Special Issue 66/3 2025 volume: 66 number: 3 startpage: 529 endpage: 533 This study investigated the presence of residues of eleven coccidiostats in unmedicated feed intended for broilers in the withdrawal phase, with the aim of assessing potential cross- contamination during feed production. Between 2023 and 2024, a total of 341 feed sam- ples were analysed, using validated LC-MS/MS methodology, for amprolium, diclazuril, halofuginone, lasalocid, salinomycin, maduramycin, monensin, narasin, nicarbazin, robe- nidine, semduramycin. Detected concentrations ranged from below the limit of quantifica- tion to 85.0 mg/kg. Non-compliant samples were identified for diclazuril (2/243), lasalocid (1/328), salinomycin (12/329), maduramycin (11/324), monensin (6/324), narasin (6/315), nicarbazin (2/327), and robenidine (7/333). The highest detected concentrations per analyte were: diclazuril 0.133 mg/kg, lasalocid 1.33 mg/kg, salinomycin 85.0 mg/kg, maduramycin 0.834 mg/kg, monensin 25.30 mg/kg, narasin 58.15 mg/kg, nicarbazin 33.70 mg/kg, and robenidine 35.2 mg/kg. Amprolium, halofuginone, semduramycin were not detected in any feed sample. Overall, 42 out of 341 feed samples (12.3%) exceeded the maximum permitted levels, indicating insufficient cleaning of production lines and inadequate separation be- tween medicated and non-medicated feed. The occurrence of such residues in feed intended for broilers in the withdrawal phase poses a risk of non-compliant residues in edible tissues, highlighting the need for stricter hygiene protocols, improved quality control in feed mills, and continuous monitoring to safeguard food safety and public health. Coccidiostat, Residue, Feed, Carry-over, Broiler, Cross-contamination info:eu-repo/semantics/article http://creativecommons.org/licenses/by/4.0/legalcode 2025-12-01 application/pdf 393882 bytes Coccidiostat residues in withdrawal feed for broilers – implications of cross-contamination in feed production lines