o:8567 Impact of New Clonal Rootstocks on Fruit weight, yield, and Mineral Composition in Leaves and Fruits of European Plum (Prunus domestica L.), Assessed by Multivariate Analysis en Abstract: This study aimed to evaluate the influence of various rootstocks (ʻWavitʼ, ʻWeivaʼ, ʻDocera 6ʼ, ʻDospina 235ʼ and ʻMyrobalanʼ) on yield, fruit weight and the mineral composition of fruits and leaves in European plum (Prunus domestica L.) cultivars ʻČačanska Lepoticaʼ and ʻJojoʼ over three growing seasons (2021–2023). Mineral content in fruit and leaves was analysed using standard procedures (Kjeldahl, colorimetric, flame photometry and atomic absorption methods) and the DOP index was calculated to assess nutrient status. Multivariate analysis was used to assess the relationships between rootstocks, yield, fruit weight and nutrient composition in fruits and leaves. Significant variations in yield and fruit weight and nutrient content were observed among rootstocks, cultivars and years with notable interactions. ʻMyrobalanʼ and ʻDospina 235ʼ enhanced yield, while ʻDocera 6ʼ produced the largest fruit but the lowest productivity. Rootstocks influenced the macro- and microelement composition, with ʻDocera 6ʼ increasing N and P content and ʻWeivaʼ enhancing K and Cu levels. Multivariate analysis revealed distinct nutrient dynamics and productivity patterns, with ʻMyrobalanʼ linked to high productivity and balanced macroelement levels. This study highlights the critical role of rootstocks in optimizing yield, fruit quality, and nutrient balance in European plum cultivars. ʻMyrobalanʼ and ʻWeivaʼ are suited for enhancing yield and fruit quality, while ʻDocera 6ʼ and ʻDospina 235ʼ may require specific nutrient management for balanced productivity. Further research should explore the long-term effects of rootstock selection on tree performance, sustainability, and climate change adaptability. Prunus domestica L. · Clonal rootstocks · Fruit macro- and micronutrients · Leaf macro- and micronutrients · DOP index 1552099 10.1007/s42729-025-02834-w 2025-12-02T11:59:37.464Z 44 no 46 Nebojša Milošević 46 Ivana Glišić 46 Milena Đorđević 46 Sanja Radičević 46 Slađana Marić 46 Mira Milinković Institute of Soil Science 0000-0001-5175-6842 46 Tomo Milošević application/pdf 551368 https://unilib.phaidrabg.rs/o:8567 no yes 1 70 92000001 71A05 Journal of Soil Science and Plant Nutrition 2025