o:8367 ECO-FRIENDLY ULTRASOUND EXTRACTION OF PHENOLICS FROM MENTHA PIPERITA: EFFECT OF KEY PARAMETERS en Ultrasound-assisted extraction (UAE) is an efficient and environmentally friendly technique for extracting valuable bioactive compounds from plant materials. Mentha piperita L., known for its distinctive aroma, therapeutic potential, and rich polyphenol profile, is a promising candidate for green extraction methods (1). In this study, the influence of ultrasonic amplitude, extraction time, and pulse mode on the yield of phenolic compounds relevant for both health and natural preservation is investigated. Aerial parts of Mentha piperita L. were extracted with an ultrasonic probe at three amplitudes (20%, 60%, 100%) and five extraction times (2–10 min), totalling 15 UAE conditions. Pulsed ultrasound was also tested. The extractions were performed with 50% EtOH (S/L 1:20), and the phenolic compounds were quantified by HPLC-DAD. The phenolic content increased with both amplitude and time. The highest yields were obtained at 100% amplitude and 10 min: 10.13 μg/mL caffeic acid, 17.05 μg/mL catechin, 44.57 μg/mL epicatechin, and 674.52 μg/mL rosmarinic acid. The pulsed mode resulted in a slightly lower extraction efficiency compared to continuous ultrasound. High-intensity, long-duration UAE significantly enhances the extraction of phenols from Mentha. Continuous ultrasound is more effective than the pulsed mode, probably due to more uniform cavitation and better destruction of cell walls. 1552099 10.60738/ah73-k927 2025-10-20T11:42:54.915Z 44 no 46 Zorana Mutavski Institute for Medicinal Plant Research ''Dr. Josif Pančić'' Belgrade 0000-0002-6998-0292 46 Jelena Jeremić Faculty of Technology Novi Sad, University of Novi Sad, Serbia 46 Nataša Nastić Faculty of Technology Novi Sad, University of Novi Sad, Serbia 46 Krunoslav Aladić Faculty of Food Technology Osijek, University of Josip Juraj Strossmayer in Osijek, Croatia 46 Stela Jokić Faculty of Food Technology Osijek, University of Josip Juraj Strossmayer in Osijek, Croatia 46 Senka Vidović Faculty of Technology Novi Sad, University of Novi Sad, Serbia application/pdf 1961563 https://unilib.phaidrabg.rs/o:8367 no yes 1 70 92000004 71A10 ABSTRACTS BOOK Polyphenols Applications 2025