o:7922 PREPARATION OF BIOPOLYMER BASED PESTICIDE AGAINST PHYTOPHTORA PATHOGENS en In this work, a new formulation that gradually released encapsulated Thuja plicata essential oil (TPEO) as an active component from a biopolymer matrix within a given period was obtained. Firstly, biopolymer matrix consisting of a chitosan-gelatin mixture was prepared. Afterwards, TPEO essential oil was encapsulated into the biopolymer matrix and an oil-in-water emulsion was formed. The main goal of this work was to obtain stable formulation by optimizing synthesis parameters (biopolymers ratio, amount of Tween 80 and homogenization time). FTIR spectra analysis confirmed that the addition of gelatine to the chitosan solution contributed to the forming of strong electrostatic interaction between these polymers, and also revealed hydrogen interactions between active components of TPEO (methyl thujate) and polymer chains. By optimizing biopolymers ratio and amount of Tween 80, nanoemulsion with average droplet size around 150 nm was obtained. The stability of the emulsions was confirmed by zeta potential measurements, with the value of about 30 mV, even after 14 days of ageing at room temperature. 1552100 978-86-80109-27-5 91552100 170258441 2025-06-30T10:29:24.262Z 44 no 46 Jovana Ćirković University of Belgrade, Institute for Multidisciplinary Research, Belgrade 46 Aleksandar Radojković Center of Excellence for Green Technologies, University of Belgrade, Institute for Multidisciplinary Research, Belgrade 46 Jelena Jovanović University of Belgrade, Institute for Multidisciplinary Research, Belgrade; Center of Excellence for Green Technologies, University of Belgrade, Institute for Multidisciplinary Research, Belgrade 46 Sanja Perać University of Belgrade, Institute for Multidisciplinary Research, Belgrade 46 Zorica Branković University of Belgrade, Institute for Multidisciplinary Research, Belgrade; Center of Excellence for Green Technologies, University of Belgrade, Institute for Multidisciplinary Research, Belgrade 46 Ivan Milenković University of Belgrade, Faculty of Forestry, Belgrade, Serbia; Mendel University in Brno, Faculty of Forestry and Wood Technology, Zemědělská Brno, Czech Republ 46 Slobodan Milanović University of Belgrade, Faculty of Forestry, Belgrade, Serbia; Mendel University in Brno, Faculty of Forestry and Wood Technology, Zemědělská Brno, Czech Republ 46 Jovan Dobrosavljević University of Belgrade, Faculty of Forestry, Belgrade 46 Vanja Tadić Institute for Medicinal Plant Research ''Dr. Josif Pančić'' Belgrade 0000-0003-1761-604X 46 Ana Žugić Institute for Medicinal Plant Research ''Dr. Josif Pančić'' Belgrade 0000-0002-7722-6169 46 Goran Branković University of Belgrade–Institute for Multidisciplinary Research, Belgrade; Center of Excellence for Green Technologies, University of Belgrade, Institute for Multidisciplinary Research, Belgrade application/pdf 793162 https://unilib.phaidrabg.rs/o:7922 no yes 1 70 92000004 71A10 BOOK OF ABSTRACTS 8th Conference of the Serbian Society for Ceramic Materials 56 56 8th Conference of the Serbian Society for Ceramic Materials 2025