o:7478 COMPARATIVE CHEMICAL STUDY OF THREE JUNIPERUS SPECIES en Plant-derived products and their compounds have been used in traditional medicine since early times. Recent works report promising potential regarding the use of plants in the treatment and/or prevention of several hardto- cure diseases, such as atherosclerosis, cancer, cardiovascular diseases, diabetes, and neurological disorders, among others. The genus Juniperus has been recognized as the source of valuable active compounds. This genus includes roughly 68 species and 36 varieties and belongs to the Cupressaceae family. This work gave insight into differences in chemical composition and antioxidative properties of three Juniperus sp., namely J. communis L. (common juniper), J. oxycedrus L. (prickly juniper) and J. sibirica L. (Siberian juniper). Common juniper (J. communis L.), based on the information provided by EMA monograph and PDR, is traditionally used to treat renal suppression, acute and chronic cystitis, bladder catarrh, albuminuria, leucorrhea, and amenorrhea. These uses are mainly attributed to its bioactive compounds belonging to different secondary metabolite groups, such as polyphenols, terpenoids, organic acids, flavonoids, tannins. While the data on common juniper are abundant, the investigations considering the prickly and Siberian juniper are scarce. The folium and ripe pseudofructus from all three species were collected from a locality in Montenegro (Plav, the mountain Čakor) in August 2019. The extraction method was maceration, performed using 70% EtOH, the plant material was ground until the particle size was 0.7 mm, while the drug:extract ratio was kept to be 1:5. To determine total phenolics, Folin–Ciocalteu (FC) spectrophotometric method was applied, while total procyanidins and tannins were estimated by following the procedure described in 10th European Pharmacopoeia. The antioxidant capacity of extracts was assessed by the DPPH and FRAP assays. The obtained results (Table 1) pointed out the differences between the investigated species. While the TP content was similar in J. communis and J. oxycedrus, J. sibirica although less abundant in tannin and polyphenolic content, exhibited significant antioxidant properties, comparable to control (BHT). Juniperus sp., maceration, chemical and antioxidant properties. 1552100 978-86-7520-629-3 2025-04-16T10:57:43.295Z 44 no 46 Vanja Tadić Institute for Medicinal Plant Research ''Dr. Josif Pančić'' Belgrade 0000-0003-1761-604X 46 Ana Žugić Institute for Medicinal Plants Research "Dr Josif Pančić" Belgrade 0000-0002-7722-6169 46 Dragica Bojović Faculty for Food Technology, Food Safety and Ecology, University of Donja Gorica, Podgorica, CG 46 Miomir Šoškić 46 Ivana Nešić Univerzitet u Nišu, Medicinski fakultet, Niš application/pdf 1209468 https://unilib.phaidrabg.rs/o:7478 no yes 1 70 92000004 71A10 BOOK OF ABSTRACTS PTEP 2025 73 74 NINTH INTERNATIONAL CONFERENCE SUSTAINABLE POSTHARVEST AND FOOD TECHNOLOGIES INOPTEP 2025 and XXXVII NATIONAL CONFERENCE PROCESSING AND ENERGY IN AGRICULTURE 2025