o:7477 ENCAPSULATION OF CANNABIDIOL-RICH HEMP EXTRACT en Hemp is grown as an agricultural crop, characterized by low content of psychoactive Δ-9 tetrahydrocannabinol (THC, less than 0.3%). Numerous research caused the increase in scientific knowledge regarding hemp composition and health benefits, whereby much attention has been focused on non-psychoactive cannabinoids, especially cannabidiol (CBD) that exert potent anti-inflammatory, and neuro-protective effects. These findings induced an intense growth in utilization of CBD-based nutritional supplements and cosmetics. Recently, supercritical extraction with carbon dioxide (SCO2) was shown to extract notable amounts of CBD from hemp compared to the conventional extractions. However, CBD possesses demanding physicochemical characteristics, such as sensitivity against light, rapid degradation and poor water solubility. These features make CBD a suitable candidate for encapsulation into advanced delivery systems (DSs). In the current investigation, SCO2 extraction was used for the production of CBD-rich hemp extract using inflorescence of Cannabis sativa L., Cannabaceae (var. Helena). Comprehensive chemical profiling of the obtained hemp extract was achieved by HPLC analysis. Afterwards, hemp extract was encapsulated into nanosized DSs that were characterized by determination of mean diameter, polydispersity index (PDI) and zeta potential (assessed by photon correlation spectroscopy, PCS), encapsulation efficiency, as well as pH and conductivity of the obtained dispersion of nano-DSs. To test the preliminary physicochemical stability of the obtained nano-DSs, PSC measurements, as well as pH and conductivity measurements were repeated after 1 month of room temperature storage. CBD content was 13.02 % in the obtained hemp extract. Encapsulation efficiency of CBD in the nano-DSs was quite high and amounted 99.99%. Their size was 243.30±1.77 nm, with low PDI of 0.213±0.016 indicating uniform particle distribution, while zeta-potential was 65.53±2.65 suggesting good kinetic stability. pH value was 4.62, while conductivity was 423 μs/cm. Repeated measurements of size, PDI, zeta potential, pH and conductivity did not change significantly compared to the initial measurements, indicating satisfactory preliminary physico-chemical stability of the developed nano encapsulants of CBD-rich hemp extract. In conclusion, hemp extract rich in CBD was successfully encapsulated into nano-sized DSs which were proven to enable satisfactory preliminary physico-chemical stability of CBD, otherwise sensitive to environmental conditions. Further investigations of biological activities of the developed nano-vehicles are needed to enable their potential application in the food, cosmetic and pharmaceutical industry. supercritical carbon dioxide extraction, nano-sized delivery systems, preliminary physico-chemical stability 1552100 978-86-7520-629-3 2025-04-16T10:34:09.949Z 44 no 46 Ana Žugić Institute for Medicinal Plant Research ''Dr. Josif Pančić'' Belgrade 0000-0002-7722-6169 46 Vanja Tadić Institute for Medicinal Plants Research "Dr Josif Pančić" Belgrade 0000-0003-1761-604X 46 Marija Tasić Kostov University of Nis, Faculty of Medicine, Nis 46 Ivana Nešić University of Nis, Faculty of Medicine, Nis application/pdf 1194761 https://unilib.phaidrabg.rs/o:7477 no yes 1 70 92000004 71A10 BOOK OF ABSTRACTS PTEP 2025 71 72 NINTH INTERNATIONAL CONFERENCE SUSTAINABLE POSTHARVEST AND FOOD TECHNOLOGIES INOPTEP 2025 and XXXVII NATIONAL CONFERENCE PROCESSING AND ENERGY IN AGRICULTURE 2025