o:6230 Optimisation of oregano essential oil encapsulation using fluidized bed granulation en The essential oil (EO) from the aerial parts in the flowering phase of Origanum heracleoticum L. (OEO), has been traditionally used in treating urinary tract infections, bronchitis, rheumatic diseases, and gastroenteritis. Beyond antimicrobial properties, OEO, rich in phenols thymol and carvacrol, showed also antioxidant proprieties. Food industries are looking for natural antimicrobial agents and antioxidants to replace synthetic ones due to health reasons. EOs are natural products that can satisfy that need, but their composition and activity could change during thermal storage conditions. The aim of this study is to optimize the process of OEO encapsulation using fluidized bed granulation (FBG). Our study monitored the effects of various factors (type of carrier, temperature in the fluid bed, and flow rate of the sprayed o/w emulsion of OEO with carrier solids) on the yield and encapsulation efficiency. The highest yield and encapsulation efficiency were obtained with carboxymethylcellulose sodium as a carrier, average temperature of less than 35° C, and a 10 ml/min flow rate of OEO emulsion. The number of total phenols in prepared samples was determined using GC-MS and spectrophotometrically at 275 nm after FBG, and in selected samples after nine months of storage at ambient temperature. It was observed that fluidized bed granules had good stability. Our results showed that FBG, as a one-step drying process with gentle treatment of temperature-sensitive substances, is suitable for volatile substances such OEO. origanum heracleoticum, essential oil, encapsulation, fluidized bed granulation 1552100 978-86-7994-063-6 2024-11-27T12:37:05.965Z 44 no 46 Dubravka Bigović Institute for Medicinal Plant Research, ''Dr. Josif Pančić'', Belgrade 0000-0003-1999-2743 46 Snežana Kuzmanović Nedeljković Institute for Medicinal Plants Research "Dr Josif Pančić", Belgrade 0000-0003-4989-8758 46 Nada Ćujić Nikolić Institute for Medicinal Plants Research "Dr Josif Pančić", Belgrade 0000-0002-6736-3637 46 Zorana Mutavski Institute for Medicinal Plant Research, ''Dr. Josif Pančić'' Belgrade 0000-0002-6998-0292 46 Slađana Pilipović Institute for Medicinal Plant Research "Dr.Josif Pančić", Belgrade 46 Milica Radan Institute for Medicinal Plant Research, ''Dr. Josif Pančić'' Belgrade 0000-0002-6727-7624 46 Katarina Šavikin Institute for Medicinal Plant Research, ''Dr. Josif Pančić'' Belgrade 0000-0002-2086-9593 46 Darko Jaćimovski Institute of Chemistry, Technology and Metallurgy, Belgrade application/pdf 2688317 https://unilib.phaidrabg.rs/o:6230 no yes 1 70 92000004 71A10 e-ABSTRACT BOOK 15 15 FoodTech2024 2024