o:6197 Thymus serphyllum and th. vulgaris essential oil and hydrolate as a potent antioxidants and a-glucosidase inhibitors en Thymus serphyllum L. (wild thyme) and Th. vulgaris (common thyme) are well-known medicinal and culinary herbs with a significant amount of essential oil, rich in bioactive phenolic terpenes. The aim of this study was to investigate the chemical composition, antioxidant and antidiabetic effects of essential oils (EO) and hydrolates (HYD) of two Thymus species grown in the fields of the Institute for Medicinal Plants Research "Dr. Josif Pančić“, Pančevo, Serbia. Wild thyme contained mainly bornyl acetate (almost 50% of EO) and thymol (even 98% of HYD). Common thyme was characterized by high amounts of thymol (over 50% and 90%, respectively). The results of the Folin-Cioacalteu method show that the EO of both species have a total phenolic content of over 350 mg GAE/g, while the HYD displayed values of approximately half of that. The total reduction power test results show that the EO of both species has dose-dependent antioxidant activity, reaching values of about 170 µM AAE/g at the highest concentration applied, while the values for HYD are about 125 µM AAE/g. The EO of both species inhibited α-glucosidase in a dose-dependent manner and achieved an inhibition of about 80% at the highest applied concentration. These findings underscore the significant presence of phenolic compounds with potent antioxidant and antidiabetic properties in EO and HYD of both thyme species, thereby highlighting their potential applications in the food and pharmaceutical industries. Thymus, essential oil, hydrolate, antioxidant, antidiabetic 1552100 978-86-7834-438-1 2024-11-26T11:54:56.405Z 44 no 46 Ana Alimpić Aradski University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac" Belgrade 46 Mariana Oalđe Pavlović University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac" Belgrade 46 Smiljana Janković University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac" Belgrade 46 Dejan Pljevljakušić Institute for Medicinal Plant Research, ''Dr. Josif Pančić'' Belgrade 0000-0003-2362-8290 46 Katarina Šavikin Institute for Medicinal Plant Research "Dr.Josif Pančić", Belgrade 0000-0002-2086-9593 46 Sonja Duletić-Laušević University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac" Belgrade application/pdf 1583622 https://unilib.phaidrabg.rs/o:6197 no yes 1 70 92000004 71A10 BOOK OF ABSTRACTS 66 66 UNIFood2024 Conference 3rd International UNIfood Conference 2024