o:607 The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization en Abstract: This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC® 7469™). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 °C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 ± 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 ± 7.6 mg glucose/g dm extract), and α-amino nitrogen 80% (161.2 ± 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry. Sažetak Spent coffee grounds, Fermentation, Polyphenols, Antioxidants, Enzymes, Lactic acid bacteria 1552099 10.1007/s13399-021-01605-8 2022-09-08T11:27:17.776Z 44 yes 46 Marija Milić Faculty of Technology and Metallurgy, University of Belgrade 46 Aneta Buntić Institute of Soil Science 0000-0002-7068-1804 46 Katarina Mihajlovski Faculty of Technology and Metallurgy, University of Belgrade 46 Nevena Ilić Faculty of Technology and Metallurgy, University of Belgrade 46 Slađana Davidović Faculty of Technology and Metallurgy, University of Belgrade 46 Suzana Dimitrijević-Branković Faculty of Technology and Metallurgy, University of Belgrade application/pdf 132068 https://unilib.phaidrabg.rs/o:607 no yes 1 70 92000001 71A05 Biomass Conversion and Biorefinery 2021