o:593 Changes in Eutric Cambisol due to long-term mineral fertilisation: A case study in Serbia en Abstract: The paper presents the results of a 50-year fertilisation experiment on Eutric Cambisol with increasing doses of nitrogen and constant doses of phosphorus and potassium. The changes in main parameters of soil fertility were compared with the initial level at the beginning of the experiment in 1963 (baseline), as well as with the adjacent natural meadow soil. The long-term application of mineral fertilisers without replenishment of calcium resulted in strong acidification, an increase in clay content, an increase in the number of fungi, and a decrease in the number of ammonifiers and oligonitrophiles. In the fertilised treatments, an increase in the content of plant-available phosphorus and potassium and a significant decrease in the plant available boron and zinc with an increase in the N dose were observed. Long-term addition of high doses of N harmed the total number of microflora, actinomycetes, ammonifiers, and oligonitrophiles compared to untreated meadow soil. Significant changes in soil’s physical and chemical properties resulted in the transformation of Eutric Cambisol into Dystric Cambisol. Inappropriate agricultural practices, such as adding only mineral fertilisers, can lead to significant soil degradation even on the sites with favourable bioclimatic conditions for cropping. The results showed that causal chains between microbiological and chemical parameters need to be better researched and understood in the future. This and other long-term experimental results should be used to calibrate agro-ecosystem models in Serbia. Nitrogen; acidification; nutrients; clay; degradation; soil microflora 1552099 10.4081/ija.2022.2029 2022-09-08T08:38:56.462Z 44 yes 46 Nikola Koković Institute of Soil Science 0000-0002-0366-916X 46 Goran Jačimović University of Novi Sad, Faculty of Agriculture 46 Biljana Sikirić Institute of Soil Science 0000-0002-8308-4582 46 Vladimir Čirić University of Novi Sad, Faculty of Agriculture 46 Vladan Ugrenović Institute of Soil Science 0000-0001-6642-8235 46 Aigul Zhapparova Kazakh National Agrarian Research University 46 Elmira Saljnikov Institute of Soil Science;Mitscherlich Academy for Soil Fertility (MITAK) 0000-0002-6497-2066 application/pdf 802813 https://unilib.phaidrabg.rs/o:593 no yes 17 70 92000001 71A05 Italian Journal of Agronomy 17:2029 2022