
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/">
  <dc:publisher>MDPI</dc:publisher>
  <dc:format>application/pdf</dc:format>
  <dc:format>1243967 bytes</dc:format>
  <dc:creator id="https://orcid.org/0000-0002-7462-310X">Cvetković, Vladimir J.</dc:creator>
  <dc:creator id="https://orcid.org/0000-0001-9628-3882">Mitić, Zorica S.</dc:creator>
  <dc:creator id="https://orcid.org/0000-0003-1694-3823">Stojanović-Radić, Zorica</dc:creator>
  <dc:creator id="https://orcid.org/0000-0003-4570-4370">Matić, Sanja Lj.</dc:creator>
  <dc:creator id="https://orcid.org/0000-0002-2436-8294">Nikolić, Biljana M.</dc:creator>
  <dc:creator id="https://orcid.org/0000-0002-8736-6771">Rakonjac, Ljubinko</dc:creator>
  <dc:creator id="https://orcid.org/0000-0002-3407-6498">Ickovski, Jovana</dc:creator>
  <dc:creator id="https://orcid.org/0000-0002-3128-6510">Stojanović, Gordana</dc:creator>
  <dc:description xml:lang="eng">Abstract: Essential oils (EOs) derived from evergreen conifer trees are recognized as complex sources
of agents with various biological activities. In this study, the biological activities of the EOs derived
from Chamaecyparis lawsoniana (A.Murray bis) Parl. (CLEO) and Thuja plicata Donn ex D.Don (TPEO)
were investigated. The third instar larvae of the fruit fly Drosophila melanogaster were used as a
model organism to determine the in vivo toxicity, genotoxicity, and antigenotoxicity of five different
concentrations of CLEO and TPEO, ranging from 0.19% to 3%. Artemia salina was used for acute
toxicity assessment and determination of LC50 after 24 h and 48 h. The antimicrobial activity
of the CLEO and TPEO was tested against fourteen bacterial and two fungal strains using the
microdilution method. The larvicidal activity and developmental time of D. melanogaster increased as
the concentrations of the EOs rose. CLEO and TPEO produced a dose-dependent increase in DNA
damage compared with the negative control. Both oils, at concentrations up to 1.5%, demonstrated
the ability to reduce the genotoxic effect induced by ethyl methanesulfonate, with a reduction rate
exceeding 60%. CLEO and TPEO were highly toxic against Artemia salina nauplii. The results indicate
that the tested EOs act as antimicrobial agents against some pathogenic bacteria and fungi. CLEO
and TPEO show promising potential as a source of antimicrobial and antigenotoxic agents and as
potential biocides against pest insects and arthropods.</dc:description>
  <dc:source>Forests</dc:source>
  <dc:source>volume: 15</dc:source>
  <dc:source>startpage: 69</dc:source>
  <dc:language>eng</dc:language>
  <dc:identifier>https://unilib.phaidrabg.rs/o:5817</dc:identifier>
  <dc:identifier>doi:10.3390/f15010069</dc:identifier>
  <dc:identifier>ISSN: 1999-4907</dc:identifier>
  <dc:date>2024</dc:date>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:subject xml:lang="eng">Keywords: essential oil; Artemia salina; toxicity; genotoxicity; antigenotoxicity; Drosophila melanogaster; biopesticides</dc:subject>
  <dc:title xml:lang="eng">Biological Activities of Chamaecyparis lawsoniana (A.Murray bis) Parl. and Thuja plicata Donn ex D.Don Essential Oils: Toxicity, Genotoxicity, Antigenotoxicity, and Antimicrobial Activity</dc:title>
</oai_dc:dc>