o:5503 In vitro spironolactone permeation study: role of different alkyl polyglucoside emulsifiers and glycolic acid en The skin is the largest human organ covering the entire body. It is composed of layers of cells that act like a barrier and keep substances from permeating and entering the systemic circulation. The release of the active substance from vehicle is the first important step in achieving an adequate effect of the drug. In case of topical application, it should permeate through the skin in order to achieve a local effect, avoiding systemic absorption (Hadgraft et al., 2005). Franz diffusion cells are a widely used methodology to evaluate in vitro drug permeation (Bielfeldt et al., 2022). Recently, spironolactone (SP) was introduced as off-label topical acne therapy, in order to reduce side effects when administered orally (Salama et al., 2020). Alkyl polyglucoside (APG)-based emulsions with 5% of SP showed acceptable skin irritation profiles, which indicate that these emulsions could be used as prospective carriers for off-label topical SP (Ilic et al., 2021). This work’s main objective was to examine the SP permeation through skin from different APG-based topical emulsions with or without glycolic acid (GA) as excipient, using Franz diffusion cells. 1552101 1857 - 8969 1552099 10.33320/maced.pharm.bull.2023.69.03.066 2024-10-01T07:06:56.841Z 44 no 46 Dušan Ilić University of Niš-Faculty of Medicine, Department of Pharmacy 46 Maja Cvetković University of Niš-Faculty of Medicine, Department of Pharmacy 46 Ana Žugić Institute for Medicinal Plant Research “Dr. Josif Pančić” 0000-0002-7722-6169 46 Slavica Sunarić University of Niš-Faculty of Medicine, Department of Chemistry 46 Marija Tasić-Kostov University of Niš-Faculty of Medicine, Department of Pharmacy application/pdf 2055028 https://unilib.phaidrabg.rs/o:5503 no yes 1 70 92000004 71A10 Македонски Фармацевтски билтен Macedonian Pharmaceutical Bulletin 69 135 136 2023