The skin is the largest human organ covering the entire body. It is composed of layers of cells that act like a barrier and keep substances from permeating and entering the systemic circulation. The release of the active substance from vehicle is the first important step in achieving an adequate effect of the drug. In case of topical application, it should permeate through the skin in order to achieve a local effect, avoiding systemic absorption (Hadgraft et al., 2005). Franz diffusion cells are a widely used methodology to evaluate in vitro drug permeation (Bielfeldt et al., 2022). Recently, spironolactone (SP) was introduced as off-label topical acne therapy, in order to reduce side effects when administered orally (Salama et al., 2020). Alkyl polyglucoside (APG)-based emulsions with 5% of SP showed acceptable skin irritation profiles, which indicate that these emulsions could be used as prospective carriers for off-label topical SP (Ilic et al., 2021). This work’s main objective was to examine the SP permeation through skin from different APG-based topical emulsions with or without glycolic acid (GA) as excipient, using Franz diffusion cells. In vitro spironolactone permeation study: role of different alkyl polyglucoside emulsifiers and glycolic acid info:eu-repo/semantics/conferenceProceedings All rights reserved Македонски Фармацевтски билтенMacedonian Pharmaceutical Bulletin vol. 69 str. 135-136 2023 Ilić, Dušan Cvetković, Maja Žugić, Ana Sunarić, Slavica Tasić-Kostov, Marija application/pdf 2055028 bytes https://unilib.phaidrabg.rs/o:5503 doi:10.33320/maced.pharm.bull.2023.69.03.066 ISSN: 1857 - 8969 eng