
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/">
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject xml:lang="eng">Keywords: Enterobacteiaceae; ciprofloxacin-resistant; qnrA; qnrB; qnrS; genes</dc:subject>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:identifier>https://unilib.phaidrabg.rs/o:5419</dc:identifier>
  <dc:identifier>doi:10.3390/antibiotics9100698</dc:identifier>
  <dc:description xml:lang="eng">Abstract: Excessive use of antimicrobials and inadequate infection control practices has turned
antimicrobial resistance (AMR) into a global, public health peril. We studied the expression of qnrA,
qnrB, and qnrS plasmid in ciprofloxacin (CIP)-resistant strains of Escherichia coli in swine and humans
from Romania, using the Polymerase Chain Reaction (PCR) technique. Antibiotic Susceptibility
Testing (AST) for human subjects (H) on 147 samples and 53 swine (S) was ascertained as well
as the isolation of bacterial DNA (E. coli) as follows: bacteriolysis, DNA-binding, rinsing, elution,
amplification, and nucleic acids’ migration and U.V. visualization stages. From 24 samples of E. coli
resistant to CIP collected from H subjects and 15 from S, for PCR analysis, 15 H and 12 S were used,
with DNA purity of 1.8. The statistically analyzed results using the Crosstabs function (IBM SPSS
Statistics-Ver. 2.1.), revealed the qnrS (417 bp) gene in 13 human subjects (52.0%), as well as in all
swine samples studied. The qnrB (526 bp) gene was exposed in 9 of the human patients (36.0%)
and in all swine isolates, and the qnrA (516 bp) gene was observed only in 3 of the isolates obtained
from human subjects (12.0%) and was not discovered in pigs (p &gt; 0.05). The presence of plasmids
qnrA, qnrB, and qnrS in the human samples and of qnrB and qnrS in swine, facilitates the survival of
pathogens despite the CIP action. The long-term use of CIP could cause a boost in the prevalence of
qnr resistance genes, and resistance in the pigs destined for slaughter, a perturbing fact for public
health and the human consumer.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:format>286506 bytes</dc:format>
  <dc:language>eng</dc:language>
  <dc:creator>Doma, Alexandru O</dc:creator>
  <dc:creator>Popescu, Roxana</dc:creator>
  <dc:creator>Mitulețu, Mihai</dc:creator>
  <dc:creator>Muntean, Delia</dc:creator>
  <dc:creator>Dégi, János</dc:creator>
  <dc:creator>Boldea, Marius V.</dc:creator>
  <dc:creator>Radulov, Isidora</dc:creator>
  <dc:creator>Dumitrescu, Eugenia</dc:creator>
  <dc:creator>Muselin, Florin</dc:creator>
  <dc:creator id="https://orcid.org/0000-0002-5500-7010">Puvača, Nikola</dc:creator>
  <dc:creator>Cristina, Romeo T.</dc:creator>
  <dc:date>2020-10-14</dc:date>
  <dc:title xml:lang="eng">Comparative Evaluation of qnrA, qnrB, and qnrS Genes in Enterobacteriaceae Ciprofloxacin-Resistant Cases, in Swine Units and a Hospital from Western Romania</dc:title>
  <dc:source>Antibiotics</dc:source>
  <dc:source>vol. 9</dc:source>
  <dc:source>br. 10</dc:source>
  <dc:source>str. 698-698</dc:source>
</oai_dc:dc>