o:5406 Is ECLIA Serum Cortisol Concentration Measurement, an Accurate Indicator of Pain Severity in Dogs with Locomotor Pain en Summary: Serum cortisol level reflects the activity of stress axis, ethological alterations, acute and chronic pain, life quality, or psychogenic stress. Although it is stated that stress can produce a measurable influence on the cortisol level, a certified value of this pain biomarker in dogs was not generally accepted yet. This interdisciplinary research emerged from the need for information in this field since not many studies were focused mainly on comparative analysis. We consider this field as a hot topic with various possible applications. Our survey is a methodological study within the fields of behavior and veterinary sciences, being relevant for the dog’s pain assessment. Results are having the guarantee of the high standard analysis of serum specimens, and using the updated Cortisol assay, with serum cortisol determined by Electrochemiluminescence immunoassay. We consider that our work could refresh the information in this field. Yet, an area of interest, specific pathology and pain particularities in a dog, being studied more and more in the last decade. What we can say is that serum cortisol limits cannot be adopted as a single and accurate pain marker in dog species, our study confirming these values, as non-conclusive for the assessment of the real pain levels in the dogs. Keywords: dog; pain level; cortisol value; significance; ECLIA 1552099 10.3390/ani10112036 2024-09-25T07:36:19.679Z 44 yes 46 Adela Katalin Markovszky Corinna Weber person Otília Biksi person Mihai Danes person Eugenia Dumitrescu person Florin Muselin person Vincenzo Tufarelli person Nikola Puvača Fakultet za ekonomiju i inženjerski menadžment person 0000-0002-5500-7010 Romeo Teodor Cristina person 2020 application/pdf 927257 https://unilib.phaidrabg.rs/o:5406 no yes 16 70 1552253 2020-11-02 Animals 10 11 2036 2036 2020-11-04