Boros, Bianca Roman, Diana-Larisa Vulpe, Constantina Bianca Dascalu, Daniela Kovačević, Renata Stevanović, Zoran Ostafe, Vasile Isvoran, Adriana info:eu-repo/semantics/conferenceProceedings 2022 Programme of the 10th International conference on tourism and sustainable development Timişoara, May 6, 2022 Keywords: common duckweed, ecotoxicity, mining, Moldova Nouă, pollution, heavy metals. All rights reserved application/pdf 821038 bytes The effect of water, sediment and soil samples potentially polluted by former mining activities from Moldova Noua Area (Romania) on the Growth of the Common Duckweed (Lemna Minor L.) : 10th International Online Conference Tourism and Sustainable Development, Timisoara, Romania, 5-7 May (2022) eng https://unilib.phaidrabg.rs/o:5229 cobiss:134917129 Abstract: The abandoned mining dumps generated by the former copper mining activities from Moldova Nouă area represent a potential pollution threat to both human health and the environment. As the assessment of ecotoxicity is essential for the potentially polluted area, the common duckweed (Lemna minor) was used for the analysis of several sample types. Water, sediment, and soil samples were collected in October 2020, February, May and July 2021 from several sampling points, all in the area of Moldova Nouă. The effects on common duckweed of the collected samples were investigated with a growth inhibition assay which involved the exposure for 7 days of a total number of 10 duckweed fronds for each sample. The water samples were tested without diluting the samples, while the sediment and soil samples were diluted with culture media reaching a concentration of 1%. The effect of heavy metal concentration on ecotoxic effect was also assessed. None of the tested samples showed ecotoxic effects to duckweed, although some samples showed a slight reduction of green frond number. It was observed that Mn, Fe, cd, Zn and Pb affected the number of green fronds after exposure to the tested samples.