o:4833 Effectiveness of Bradyrhizobium japonicum inoculation on nodulation dynamics in Glycine max (L.) roots en Abstract: Bradyrhizobium japonicum bacteria on the intensity of nodulation during nine phenophases (R1-R7) in relation to the isoflavone content in soybean seeds. Nine domestic soybean varieties were sown in three replicates, with Bradyrhizobium japonicum inoculated and non-inoculated seeds. The different morphological characteristics of nodules, the number and mass of nodules were studied during plants’ ontogenesis. The content of individual isoflavones in the sowing seed material was determined using high performance liquid chromatography mass spectrometry (HPLC-MS). The most abundant isoflavone was acetylgenistin in the variety Galeb (2741.4 μg/g), and the highest content of all isoflavones was recorded in the variety Gorštak (8117.7 μg/g). The variety Sava of the inoculated treatment exhibited the highest average values of the nodule mass (21.1 mg) and the highest number of nodules (23.3) in the phenophase, in which 10% of pods reached full length (R4). The average values of mass and number of nodules were calculated for the entire vegetation period, being higher in the inoculated treatment. There was no positive correlation between the content of isoflavones in seeds and the number and mass of nodules. The impact of quantity of isoflavone on nodulation intensity in soybeans was not significant,, which could be related to already saturated soil with nitrogen-fixing bacteria. Bradyrhizobium japonicum; isoflavones; nitrogen fixation; nodule; soybean 1552099 10.15835/nbha52213627 2024-06-19T07:32:31.659Z 44 no 46 Vladimir Miladinović Institute for the Vegetable Crops, Department of Genetics and Vegetable Breeding Smederevska Palanka 46 Vera Popovic Institute of Field and Vegetable Crops, National Institute of the Republic of Serbia, Novi Sad 46 Dušica Delić Institute of Soil Science 0000-0002-0626-0482 46 Zora Dajic Stevanovic University of Belgrade, Faculty of Agriculture, Department of Agrobotany, Zemun-Belgrade 46 Biljana Kiprovski Institute of Field and Vegetable Crops, National Institute of the Republic of Serbia, Novi Sad 46 Stefan Kolasinac University of Belgrade, Faculty of Agriculture, Department of Agrobotany, Zemun-Belgrade 46 Maja Mikulic-Petkovsek University of Ljubljana, Biotechnical Faculty, Department of Agronomy, Ljubljana, Slovenia 46 Vladan Ugrenovic Institute of Soil Science 0000-0001-6642-8235 application/pdf 318394 https://unilib.phaidrabg.rs/o:4833 no yes 1 70 92000001 71A05 Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2024