
<ns0:uwmetadata xmlns:ns0="http://phaidra.univie.ac.at/XML/metadata/V1.0" xmlns:ns1="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0" xmlns:ns10="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0" xmlns:ns11="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0/entity" xmlns:ns12="http://phaidra.univie.ac.at/XML/metadata/digitalbook/V1.0" xmlns:ns13="http://phaidra.univie.ac.at/XML/metadata/etheses/V1.0" xmlns:ns2="http://phaidra.univie.ac.at/XML/metadata/extended/V1.0" xmlns:ns3="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/entity" xmlns:ns4="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/requirement" xmlns:ns5="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/educational" xmlns:ns6="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/annotation" xmlns:ns7="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/classification" xmlns:ns8="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/organization" xmlns:ns9="http://phaidra.univie.ac.at/XML/metadata/histkult/V1.0">
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    <ns1:identifier>o:4643</ns1:identifier>
    <ns1:title language="en">The Validation of a Method for the Determination of Chloramphenicol in Milk</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">Abstract: The liquid chromatography tandem mass spectrometry (LC-MS/MS) method with the
liquid-liquid extraction and SPE (Waters OASIS HLB column) cleaning process was validated for the
determination of chloramphenicol (CAP) residues in milk. The optimisation of the mass
spectrometry parameters was performed by injecting the CAP standard solutions into the
electrospray ion (ESI) source. The mass spectrometer was operated in the negative ESI mode. The
validation data fulfilled the requirements established in the Regulations Decisions 2020/657/EC.
During the validation process the chloramphenicol-D5 as an internal standard was used. The
obtained results indicated the good linearity (R2 &gt; 0.99) within the range of 0.1–2.0 μg/kg. The mean
recovery for spiking levels was 96.5 ±10.59%. The limit of quantification (LOQ) was 0.1 μg /kg. The
repeatability was 10.6%. The method fulfilled all the 2020/657/EC guidelines and thus can be
extended for the routine analysis of CAP residues in milk.
</ns1:description>
    <ns1:keyword language="en">Keywords: validation; chloramphenicol; LC-MS/MS; milk.</ns1:keyword>
  </ns1:general>
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    <ns1:upload_date>2024-05-28T08:19:59.037Z</ns1:upload_date>
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        <ns3:firstname>Nikola</ns3:firstname>
        <ns3:lastname>Puvača</ns3:lastname>
        <ns3:institution>Fakultet za ekonomiju i inženjerski menadžment</ns3:institution>
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        <ns3:firstname>Sanja</ns3:firstname>
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        <ns3:firstname>Vojislava</ns3:firstname>
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        <ns3:firstname>Bojana</ns3:firstname>
        <ns3:lastname>Špirović Trifunović</ns3:lastname>
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        <ns3:firstname>Dušan</ns3:firstname>
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      <ns1:date>2022</ns1:date>
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    <ns8:approbation_period>2022-02-08</ns8:approbation_period>
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  <ns12:digitalbook>
    <ns12:name_magazine language="en">The Validation of a Method for the Determination of Chloramphenicol in Milk</ns12:name_magazine>
    <ns12:volume>5</ns12:volume>
    <ns12:booklet>1</ns12:booklet>
    <ns12:from_page>697</ns12:from_page>
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    <ns12:releaseyear>2022</ns12:releaseyear>
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