o:4629 Interpreting the Interaction of Genotype with Environmental Factors in Barley Using Partial Least Squares Regression Model en Abstract: Genotype by environment interaction (GEI) is a complex problem that complicates the barley selection and breeding process. The knowledge of the relationship between cereal phenology and climatic data is important for understanding GEI and the physiological pathways responsible for the interaction effect. The grain yield of twenty winter barley genotypes in six environments was observed. Factors influencing the variability were analyzed using a linear mixed model. The partial least squares regression (PLSR) model was applied to determine the most relevant environmental variables in certain stages of development that explained GEI effects. Biplot with environmental variables explained 43.7% of the GEI. The barley was generally the most sensitive to the environmental conditions (relative humidity, maximum temperature and its variation, sun hours, and precipitation) during the anthesis and filling stage (May) which caused GEI. Temperature variables did not show significance only in the vegetative phase. Different genotypes responded differently to environmental factors. Genotypes NS-525, NS-589, and J-103 were highlighted as widely adaptable, and Zajeˇcar was a suitable and reliable location for yield testing. The GEI information presented in this paper can be useful in traditional plant breeding and future breeding programs through molecular research of crop developmental genes and examination of physiological processes in two-row barley. Hordeum vulgare; grain yield; GEI; environmental variables; phenology; PLSR 1552099 10.3390/agronomy14010194 2024-05-24T09:35:54.073Z 44 no 46 Kamenko Bratkovic Center for Small Grains and Rural Development, Save Kovacevica 31, 34000 Kragujevac, Serbia 46 Kristina Luković Institute for Vegetable Crops, Karadordeva 71, 11420 Smederevska Palanka, Serbia 46 Vladimir Perišic Institute for Vegetable Crops, Karadordeva 71, 11420 Smederevska Palanka, Serbia 46 Jasna Savic Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11030 Belgrade, Serbia 46 Jelena Maksimovic Institute of Soil Science 0000-0001-5217-3972 46 Sladjan Adžic Institute for Vegetable Crops, Karadordeva 71, 11420 Smederevska Palanka, Serbia 46 Aleksandra Rakonjac Institute for Vegetable Crops, Karadordeva 71, 11420 Smederevska Palanka, Serbia 46 Mirela Matkovic Stojšin Tamiš Research and Development Institute, Novoseljanski put 33, 26000 Pancevo, Serbia application/pdf 2879321 https://unilib.phaidrabg.rs/o:4629 no yes 1 70 92000001 71A05 Agronomy 2024