o:3931 Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia en Abstract: Targeted physiological groups of bacteria were cultivated and identified in the brackish groundwaters of Obrenovačka Banja (OB), Lomnički Kiseljak (LK) and Velika Vrbnica (VV) using biological activity reaction tests (BARTs) to assess the biocorrosion, biofouling and health risks. The highest density of iron-related, sulfate-reducing, slime-forming, facultatively anaerobic heterotrophic, denitrifying bacteria and representatives of Pseudomonas spp. was recorded in the OB sample, while the lowest density of the same physiological groups of bacteria was recorded in the LK sample. Facultatively anaerobic heterotrophic bacteria were the most abundant in the OB and LK samples, while, in contrast, heterotrophic aerobic bacteria were the most abundant in the VV sample. All tested samples were characterized by a high degree of biochemical activity associated with iron-related, sulfate-reducing, slime-forming, heterotrophic aerobic and facultatively anaerobic bacteria. Also, high biochemical activity of denitrifying bacteria was recorded in the OB sample, and the same activity of Pseudomonas species was recorded in the OB and VV samples. For OB and LK groundwaters, the highest degree of risk was estimated for biocorrosion process, while for the OB and VV occurrences, the highest degree of risk was estimated for biofouling process. The health risk was present for all examined groundwaters. Caution is warranted in further use of all investigated occurrences due to the established public health risk and an immediate revitalization of the OB, LK and VV wells is necessary. brackish groundwaters, biological activity reaction tests, Serbia 1552099 10.46793/ICCBI23.086S 2024-02-05T11:38:29.708Z 44 no 46 Vladimir Šaraba University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Molecular Genetics and Ecology of Microorganisms, Belgrade, Serbia Jasmina Nikodinović-Runić University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Molecular Genetics and Ecology of Microorganisms, Belgrade, Serbia person Vesna Obradović Jaroslav Černi Water Institute, Laboratory Jaroslav Černi, Belgrade, Serbia person 0009-0008-2157-0615 Ivica Dimkić University of Belgrade, Faculty of Biology, Chair of Biochemistry and Molecular Biology, Belgrade, Serbia person Tamara Janakiev University of Belgrade, Faculty of Biology, Chair of Biochemistry and Molecular Biology, Belgrade, Serbia person Veselin Dragišić University of Belgrade, Faculty of Mining and Geology, Department of Hydrogeology, Belgrade, Serbia person Milica Ćirić University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Laboratory for Molecular Genetics and Ecology of Microorganisms, Belgrade, Serbia person application/pdf 2094618 https://unilib.phaidrabg.rs/o:3931 no yes 1 70 92000004 71A04 2nd International Conference on Chemo and BioInformatics, Kragujevac, Serbia, September 28-29, 2023 86 90 Jovana Cvijića bb, Kragujevac, Serbia Institute for Information Technologies, University of Kragujevac, Serbia 2023