o:3845 Variability of in vitro germination of Picea omorika pollen en Serbian spruce (Picea omorika /Panc./Purkyne) is a Balkan endemic and Tertiary relict, therefore it has an exceptional significance from the historical-geographical aspect as well as from the aspect of tree im- provement and biodiversity conservation. Pollen was collected from 24 trees in two consecutive years and an- alyzed once a month. Germination of fresh pollen and pollen stored under different temperatures (room tem- perature: 23 ±1°C, +4°C, –15°C and –20°C) by in vitro method on a medium with 10% sucrose was analyzed. Fresh pollen germination was 53.63% and 68.06% for pollen collected in first and second year, respectively. After one year of storage at –20°C pollen germination was 22.36% and 60.72%, respectively. The differences between pollen collection years, temperature treatments, storage periods and individuals were statistically significant. Since pollen germination rates of individual trees between the first and the second year showed weak positive correlation, one could conclude that germination of fresh pollen grains is under the certain in- fluence of environmental conditions. It is determined strong positive correlations between fresh and stored pollen at –20°C from both years of pollen collection. " Serbian spruce pollen, pollen conservation, treatments, germination 1552101 1641-1307 1552256 2083-8387 1552099 http://dx.doi.org/10.12657/denbio.069.002 2024-01-22T08:30:58.763Z 44 yes 46 Branislava Batos 46 Biljana M. Nikolić 0000-0002-2436-8294 application/pdf 538108 https://unilib.phaidrabg.rs/o:3845 no yes 1 70 1552253 Dendrobiology 69 13 19 Polish Academy of Sciences, Institute of Dendrology 2013