o:2975 PHENOLIC PROFILE, ANTIOXIDATIVE AND ANTIMICROBIAL ACTIVITY OF „ORANGE AND BLUE SUBTYPES” OF TURKISH PROPOLIS en Introduction. Propolis is a very complex resinous mixture and exhibits many pharmacological properties. Recently, several studies identified two subtypes (“orange and blue“) of Romanian, Serbian, Croatian, German, French, and Slovenian poplar propolis using different analytical techniques. The HPTLC fingerprint of Turkish propolis samples also showed presence of two different botanical subtypes. Aim. The aim of this study was the comprehensive phenolic profiling of orange, blue, and one more subtype of Turkish poplar propolis using accurate mass spectrometry, examination of quality control parameters, as well as its antioxidative and antimicrobial activity. Methods. Phenolic profile of 48 Turkish propolis samples was investigated by using UHPLC–LTQ/Orbitrap/MS/MS method. Quality control parameters such as total phenolic (TPC) and flavonoids content (TFC), and antioxidative activity was investigated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Results. Fifty-one phenolic compounds were identified in three subtypes of Turkish propolis. Quality control parameters showed higher TPC, TFC, as well as antioxidative activity of orange subtype. Turkish propolis samples have showed a great antimicrobial potential against all tested strains, with the S. pyogenes as the most sensitive one. Conclusions. Turkish propolis, especially its orange subtype, can be considered as the high-quality subtype due to its quality, strong antioxidative and antimicrobial activity. Ferulic and caffeic acid were dominant compounds in almost all samples that had the strongest antimicrobial activity. Turkish propolis, phenolic profile, quality control parameters, antimicrobial activity 2023-10-13T10:37:47.806Z 44 no 46 Ivica Dimkić Faculty of Biology, University of Belgrade, Belgrade 46 Petar Ristivojević Innovation Centre of Faculty of Chemistry Ltd, Belgrade 46 Jelena Trifkovic Innovation Centre of Faculty of Chemistry Ltd, Belgrade 46 E. Guzelmeric Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy and Phytotherapy, Istanbul 46 Magdalena Knežević Institute of Soil Science 0000-0003-0186-3652 46 Tanja Berić Faculty of Biology, University of Belgrade, Belgrade 46 E. Yesilada Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy and Phytotherapy, Istanbul 46 Dušanka Milojković-Opsenica Faculty of Chemistry, University of Belgrade, Belgrade 46 Slaviša Stanković Faculty of Biology, University of Belgrade, Belgrade application/pdf 436328 https://unilib.phaidrabg.rs/o:2975 no yes 1 70 92000004 71A05 10th Balkan Congress of Microbiology Microbiologia Balkanica‘2017 Sofia 2017