o:2593 Natural deep eutectic solvents combined with cyclodextrins: A novel strategy for chokeberry anthocyanins extraction en Innovative eco-friendly methods based on natural deep eutectic solvents (NaDES) coupled with ultrasoundassisted extraction were employed for chokeberry anthocyanins extractions. Nine different NaDES composed of choline chloride as a hydrogen bond acceptor and organic acids (lactic, citric, malic), sugars (glucose, fructose), polyols (glycerol, 1,2-propanediol, sorbitol), and an amide (urea) as hydrogen bond donors were screened. Malic acid-containing NaDES was selected for optimization extraction conditions (time, temperature, water in NaDES) by response surface methodology. Optimal conditions for simultaneously maximizing the anthocyanins extraction (cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, total anthocyanins) were 42.7 ◦C, 90 min, and 40 % (w/w) water in NaDES. In the next stage of this study, the possibility to improve anthocyanins extraction at elevated temperatures by incorporating different concentrations of hydroxypropyl- β-cyclodextrin into selected NaDES was investigated. The extraction was improved at hydroxypropyl-β-cyclodextrin concentrations up to 3 % (w/w). To clarify the interaction of NaDES components and anthocyanins, a molecular dynamic simulation was conducted. Aronia melanocarpa Anthocyanins NaDES Hydroxypropyl-β-cyclodextrin Molecular dynamic Green extraction Chemical compounds studied in this article: Cyanidin-3-O-galactoside (PubChem CID 441699) Cyanidin-3-O-glucoside (PubChem CID 441667) Cyanidin-3-O-arabinoside (PubChem CID 91810602) Choline chloride (PubChem CID 6209) Malic acid (PubChem CID 525) Hydroxypropyl-β-cyclodextrin (PubChem CID 14049689) 1552099 10.1016/j.foodchem.2022.134816 1552101 0308-8146 2023-07-27T09:00:51.194Z 44 yes 46 Miloš Jovanović Institute for Medicinal Plants Research “Dr. Josif Pančić" 0000-0003-0039-0929 Nemanja Krgović Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0003-3611-9911 Milica Radan Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0002-6727-7624 Nada Ćujić Nikolić Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0002-6736-3637 Jelena Mudrić Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0002-6695-2907 Zorica Lazarević Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0002-0741-2337 Katarina Šavikin Institute for Medicinal Plants Research “Dr. Josif Pančić" person 0000-0002-2086-9593 application/pdf 632184 https://unilib.phaidrabg.rs/o:2593 no yes 1 70 1552253 71A10 Food Chemistry 405 134816 2023